焦磷酸测序

Pyrosequencing is a method used for DNA sequencing by incorporating fluorescent dyes into the polymerase chain reaction (PCR) process.
焰式测序是一种在聚合酶链反应（PCR）过程中加入荧光染料的DNA测序方法。
The first step in pyrosequencing is to amplify the target DNA using PCR primers.
焰式测序的第一步是使用引物扩增目标DNA。
After amplification, the pyrosequencer detects the incorporation of each nucleotide, generating a sequence signal.
扩增后，焰式测序仪检测每个核苷酸的掺入，产生序列信号。
A key advantage of pyrosequencing is its high throughput and relatively short run time compared to other sequencing technologies.
焰式测序的一个主要优点是其高通量和与其他测序技术相比更短的运行时间。
The data generated from pyrosequencing can be analyzed using specialized software for base calling and quality control.
从焰式测序产生的数据可以使用专门的软件进行碱基呼叫和质量控制分析。
In pyrosequencing, the heat released during nucleotide incorporation is measured and converted into a digital signal.
在焰式测序中，测量核苷酸掺入时释放的热量并将其转化为数字信号。
The technique has been widely used in forensic science for DNA profiling and genetic research.
这种技术在法医学中被广泛用于DNA指纹鉴定和遗传学研究。
Pyrosequencing is particularly useful for analyzing short tandem repeats (STRs) in forensic casework.
焰式测序特别适用于法医学中对短串联重复序列（STRs）的分析。
Automated pyrosequencers have greatly simplified the process, reducing errors and increasing efficiency.
自动化的焰式测序仪极大地简化了过程，减少了错误并提高了效率。
Although next-generation sequencing methods have emerged, pyrosequencing remains a reliable alternative for specific applications.
虽然新一代测序方法已经出现，但焰式测序仍然是某些特定应用的可靠选择。